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. 2010 Apr 20;285(26):20147–20154. doi: 10.1074/jbc.M110.115584

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — PEPT2 localize to phagosomes. a, GFP-PEPT2 was ectopically expressed in HEK293T cells that were then allowed to internalize latex beads to generate phagosomes. PEPT2 shows strong membrane association and localization to phagosomes. Latex bead, 0.8 μm; scale bar corresponds to 5 μm. b, localization of endogenous PEPT2 in THP1 cells. PEPT2 localization was determined in resting THP1 cells (left) and during phagocytosis of latex beads (middle and right) by confocal microscopy. Left, PEPT2 localizes to the plasma membrane and intracellular compartments in resting cells. Middle, PEPT2 is enriched in the phagocytic cup. Right, PEPT2 associates with phagosomes in THP1s. An asterisk denotes 2 μm latex beads. Scale bar corresponds to 5 μm.