FIGURE 1.

ATGL expression in macrophages and foam cells. Mouse peritoneal macrophages, human THP-1, and human monocyte-derived macrophages (HMDM) were cultivated in DMEM, 10% LPDS in the absence (control) or presence of 100 μg of acLDL/ml. Total RNA was isolated and reverse-transcribed, and mRNA expression of ATGL (A and C) and hormone-sensitive lipase (HSL) was determined by real time PCR, including murine hypoxanthine-guanine phosphoribosyltransferase or human (hu) porphobilinogen deaminase normalization (A). Untreated macrophages were arbitrarily set to 1. Data are expressed as mean values (n = 3) ± S.E. of triplicate repeats. ***, p ≤ 0.001. B, cell extracts of macrophages, foam cells (40 μg per lane), and white adipose tissue (WAT) (10 and 40 μg per lane) were resolved by SDS-PAGE. Protein expression of ATGL and hormone-sensitive lipase were analyzed by Western blotting relative to the expression of β-actin. STD, standard.