FIGURE 2.

Analysis of hydrolase activities and lipid parameters of Atgl^−/− and WT macrophages. A, Western blotting of WT and Atgl^−/− MPM using an anti-ATGL-specific antibody. B, TG. D, CE hydrolase activities were assayed in cell lysates from WT and Atgl^−/− MPM. Lipids from MPM (control) and 24-h acetylated LDL-loaded (100 μg/ml) MPM (+acLDL) were extracted. C, TG. E, (total cholesterol), FC, and CE concentrations were measured enzymatically. Data are presented as mean values (n = 3–4) ± S.E. ***, p ≤ 0.001.