FIGURE 8.

Phagocytosis in Atgl^−/− and WT mice in vivo. Fluorescein-labeled E. coli particles (200 μl) were injected into Atgl^−/− and WT mice. After 2 h, peritoneum was flushed with 10 ml of PBS, 1 mm EDTA, and macrophages were isolated and assayed for total (A) and internalized (B) fluorescence after quenching of extracellular fluorescence by trypan blue. C, representative image showing total fluorescence in WT and Atgl^−/− MPM. Original magnification, ×63. Phagocytosis of WT cells was arbitrarily set to 100%. Relative phagocytosis is presented as mean values (n = 8–9) ± S.E. of three independent experiments. *, p < 0.05.