FIGURE 6.
PrPC levels do not affect uptake of prions. A, new stable RK13 cell lines were created to express both MoPrP and the prion-resistant G118P mutant, expressing varying levels of PrPC. Tubulin probing was used to control for equal loading of cell lysate. B, cells were exposed to prions, following which cell immunoblot (passage 8) demonstrates that MoPrP cell lines develop PrPSc, whereas no PrPSc was observed in G118P lines regardless of PrPC expression levels. C, quantification of PrPC and PrPSc demonstrates that PrPC expression does not correlate with susceptibility to prion infection.