FIGURE 6.

Impaired proliferation of XIAP KO immune cells. A and B, 10⁶ PMNs (A) and splenic T cells (B) from both WT and XIAP KO mice were stimulated with LPS (5 μg/ml) and ConA (2.5 μg/ml), respectively, and the stimulatory potential was measured by [³H]thymidine incorporation. Data represent the mean of counts per min ±S.E. from three independent experiments. C, the stimulation of T cells in response to ConA treatment was analyzed by WST-1 assays, and the survival of T cells was measured by MTT assays as described under “Experimental Procedures” (D). E, both CD4 and CD8 T cells were purified by magnetic cell sorting from the spleen of WT and XIAP KO mice. Viable cells were quantified by trypan blue dye exclusion. Both CD4 (F) and CD8 (G) T cells were stimulated with LPS, ConA, and purified chlamydial cHSP60 for 48 h and analyzed by FACS as described under “Experimental Procedures.”