TABLE 1.

Detection of C. pneumoniae in the lungs of mice

Animals were infected and sacrificed at the indicated times, and DNA was prepared from their lungs. The DNA segment coding for the chlamydial ompA gene was amplified by nested PCR as described under “Experimental Procedures.” The PCR products were separated on agarose gels, which revealed the presence of 437- and 229-bp products corresponding to the VD2 and VD4 fragment of ompA gene, respectively. Index: +/+, 437/229 bp; ND, not detected.

Experimental group	Day 3 post-infection	Day 10 post-infection	Day 20 post-infection
WT control	ND	ND	ND
WT infected	+/+	+/+	+/+
XIAP KO control	ND	ND	ND
XIAP KO infected	+/+	+/+	−/+