FIGURE 10.

Effect of the α_2B-AR C terminus on β₂-AR responsiveness to Rab8(T22N) inhibition. A, effect of transient expression of Rab8(T22N) on the cell surface expression of β₂-ARα_2B-ct. HEK293 cells were transfected with α_2B-AR, β₂-AR, or β₂-ARα_2B-ct together with the pcDNA vector (control) or FLAG-Rab8(T22N). The cell surface expression of the receptors was determined by intact cell ligand binding. The data shown are percentages of the mean value obtained from cells transfected with individual receptors and the pcDNA vector and are presented as the mean ± S.E. of three experiments. *, p < 0.05 versus Ctrl. B, effect of Rab8(T22N) on the subcellular distribution of β₂-ARα_2B-ct. HEK293 cells cultured on coverslips were transfected with GFP-tagged β₂-ARα_2B-ct together with the pcDNA vector (ctrl) or Rab8(T22N). The cells were then stained with anti-p230 antibodies (1:200 dilution). Green, receptors; red, the TGN marker p230; blue, DNA staining by 4,6-diamidino-2-phenylindole (nucleus); yellow, co-localization of receptors with p230. The data are representative images of at least three independent experiments. Scale bars, 10 μm.