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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Hypertension. 2010 May 17;56(1):105–111. doi: 10.1161/HYPERTENSIONAHA.109.144642

Figure 2. Redox dependent interaction between Axl and MHC-IIB in rat aortic VSMC.

Figure 2

A. Cells were stimulated with H2O2 (300 µM). Cell lysates were immunoprecipitated with anti-Axl antibody and interacting MHC-IIB detected by immunoblotting with MHC-IIB antibody. Equal loading was confirmed with anti-Axl antibody (lower). B. SM1 and 2 do not interact with Axl. Rat aortic VSMC were stimulated with H2O2 (300 µM). To examine interaction between Axl and SM1 and 2, cell lysates were immunoprecipitated with anti-Axl antibody and immunoblotted with SM1 and 2 antibody (upper panel). Lysates show expression of SM1 and 2 in VSMC (upper panel). Equal loading was confirmed using anti-Axl antibody (lower panel). C. Cells were stimulated with Diamide (30 µM) for the indicated times. Cell lysates were immunoprecipitated with anti-Axl antibody and interacting MHC-IIB detected by immunoblotting with MHC-IIB antibody. Equal loading was confirmed with anti-Axl antibody (lower). D. Cells were stimulated with LY83583 (1 µM) for the indicated times. Cell lysates were immunoprecipitated with anti-Axl antibody and interacting MHC-IIB detected by immunoblotting with MHC-IIB antibody. Equal loading was confirmed with anti-Axl antibody (lower).