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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure1. The localization of Gsy2-GFP within wild type yeast cells.

Figure1

The wild type yeast strain BY4743 was transformed with a centromeric plasmid encoding the GSY2 open reading frame fused at the C-terminus to GFP (pJR1420-A). After overnight growth in SC-Ura medium, aliquots of cells were examined by fluorescence microscopy. The left-hand series of images shows representative fields of cells observed using differential interference contrast. The right-hand series of panels shows fluorescence images of the same fields. Three distinct types of localization pattern could be distinguished within a single cell population; well defined bright spots (spot), spots accompanied with a haze (spot+haze), and diffuse cytoplasmic staining (diffuse). The scale bar represents 10 μm.