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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure 10. Expression of non-phosphorylatable glycogen synthase mutants restores glycogen storage to yeast strain lacking glycogenin.

Figure 10

The yeast strain CC9, which lacks both isoforms of glycogenin, was transformed with constructs encoding the S650A (pWW215), S654A (pWW217), T667A (pWW218), S650D/S654D/T667D (pWW219), R579A/R580A/R582A (pWW220), mutant forms of Gsy2-GFP, wild type Gsy2-GFP (pWW214), or empty vector (pRS314). The strain EG328-1A, from which CC9 was derived, was transformed with empty vector (pRS314). Glycogen content was assessed by staining by exposing patches of cells to iodine vapor. Expression of phosphorylation site mutants of glycogen synthase, but not expression of wild type glycogen synthase or the R579A/R580A/R582A mutant, restored glycogen accumulation to glycogenin-deficient yeast.