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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure 2. Mutation of the known phosphorylation sites in glycogen synthase causes a shift from more localized to more diffuse staining.

Figure 2

The wild type yeast strain BY4743 was transformed with centromeric plasmids encoding wild type Gsy2-GFP (pJR1420-A), S650A Gsy2-GFP (pJR1420-B), S654A Gsy2-GFP (pJR1420-C), or T667A Gsy2-GFP (pWW213). The localization of each Gsy2-GFP construct was then determined by fluorescence microscopy after overnight growth in SC-Ura medium. Panel A: In each case, the percentage of cells showing fluorescence characterized as well defined bright spots (spot), spots accompanied with a haze (spot+haze), and diffuse cytoplasmic staining (diffuse) was determined. A minimum of ten fields, containing a total of at least 250 cells were counted. The results show the mean ± standard error of the mean for three separate experiments. Panel B: The left-hand series of images shows representative fields of cells observed using differential interference contrast. The right-hand series of panels shows fluorescence images of the same fields. The three phosphorylation site mutants had a more diffuse localization pattern than did wild type glycogen synthase. The scale bar represents 10 μm.