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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure 4. Deletion of REG1 alters the subcellular distribution of glycogen synthase.

Figure 4

Deletion of the REG1 gene results in increased glycogen storage but does not alter the phosphorylation state of glycogen synthase. A strain in which REG1 had been deleted (DH11-51) was transformed with wild type Gsy2-GFP (pWW214) and grown overnight in SC-Trp medium. The localization pattern of Gsy2-GFP was scored by fluorescence microscopy as described in the legend to Figure 2 and the wild type cells were strain EG328-1A (Panel A). Representative images are shown (Panel B). The left-hand image shows a field of cells observed using differential interference contrast. The right-hand panel shows fluorescence images of the same fields. In reg1 mutant cells, Gsy2-GFP had a more diffuse distribution than in wild type cells. The scale bar represents 10 μm.