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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure 5. Removal of glucose from the growth medium results in a reduction in glycogen stores and a redistribution of glycogen synthase within the cell.

Figure 5

Wild type (EG328-1A) and pcl8 pcl10 mutant (WW10) cells were transformed with vector containing Gsy2-GFP (pJR1420-A). After overnight growth in SC-Ura medium, two aliquots of each culture were taken. Cells were collected from one aliquot by centrifugation, washed twice with sterile water, resuspended in fresh SC-Ura medium lacking glucose, and incubated at 30 °C (Starved). From the other aliquot, cells were collected by centrifugation and the growth medium was saved. The cells were washed twice with sterile water, resuspended in the saved growth medium, and incubated as above (Unstarved). After 4 hours, aliquots were taken for glycogen measurement (Panel A) and determination of the subcellular localization of glycogen synthase (Panel B). Four hours of starvation resulted in significant glycogen depletion and an increase in the number of cells exhibiting the bright spot staining pattern.