Figure 7. Co-localization of glycogen synthase and glycogen phosphorylase.
Strain MB1-8, which contains a fusion between GPH1 and a yellow-fluorescent protein cassette at the GPH1 locus, was transformed with centromeric plasmids encoding wild type Gsy2-CFP (pWW216; Panel A) or S650A Gsy2-CFP (pWW221; Panel B). The subcellular localization of both glycogen synthase and glycogen phosphorylase was determined after overnight growth in SC-Trp. Glycogen synthase and glycogen phosphorylase were found to co-localize. The scale bar represents 10 μm.