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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Can J Microbiol. 2010 May;56(5):408–420. doi: 10.1139/w10-027

Figure 8. In the absence of glycogen, glycogen synthase but not glycogen phosphorylase localizes to the nucleus.

Figure 8

Gsy2-CFP (pWW216) was expressed in strain WW33-4C, in which both glycogenin genes were deleted and the GPH1-YFP fusion described in the legend to Figure 7 was present at the GPH1 locus. The subcellular distributions of glycogen synthase and glycogen phosphorylase were determined after overnight growth in SC-Trp medium (Panel A). Glycogen synthase was found to accumulate in the nucleus of the majority of cells (indicated by the arrows in the center panel) but glycogen phosphorylase did not (note absence of Gph1-YFP fluorescence at positions indicated by arrows in the lower panel). Panel B: Nuclear localization of glycogen synthase was confirmed by co-staining with Hoechst 33342 dye. The scale bar represents 10 μm.