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. Author manuscript; available in PMC: 2010 Jun 21.
Published in final edited form as: Lab Invest. 2008 Dec 29;89(2):209–221. doi: 10.1038/labinvest.2008.127

Figure 4.

Figure 4

Effect of PTK/ZK on the expression of Flt-1, Flk-1 and VEGF on HSCs. (a) Activated HSCs were treated with PTK/ZK at various concentrations overnight. HSCs were then labeled with anti-Flt-1, or anti-Flk-1, followed by anti-rabbit PE, and analyzed by flow cytometry. Surface expression of Flt-1 and Flk-1 is presented as percentages of cells positive for the receptors, and also as mean channel fluorescence (MCF) on HSCs. Data are mean±s.e. from three experiments, *P<0.05. (b) HSCs were treated with PTK/ZK at indicated concentrations and cultured in the medium containing 1% FBS for 24 h. Cell lysates were subjected to western blot for analysis of Flt-1 and Flk-1 proteins. A representative study from three experiments is shown. (c) Effect of PTK/ZK on VEGF mRNA. HSCs were treated with PTK/ZK at the indicated concentrations before measurement of VEGF mRNA expression by real-time PCR. PTK/ZK incubation results in significant reduced VEGF mRNA expression. Data are presented as mean±s.e., n = 3, *P<0.05.