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. Author manuscript; available in PMC: 2010 Jun 21.
Published in final edited form as: Lab Invest. 2008 Dec 29;89(2):209–221. doi: 10.1038/labinvest.2008.127

Figure 5.

Figure 5

Effects of PTK/ZK on VEGF-induced HSC proliferation and Akt expression. (a) HSCs were cultured on plastic dishes precoated with type I collagen. VEGF was added at the indicated concentration and cells were incubated for 48 or 72 h. HSC proliferation was measured by BrdU incorporation as described in Materials and methods section. Data are mean±s.e. from three experiments and each concentration was repeated six times in each experiment, *P<0.05. (b) PTK/ZK at various concentrations was added to HSCs with or without VEGF (10 ng/ml). The proliferation of HSCs induced by VEGF was significantly inhibited by PTK/ZK in a dose-dependent manner. Data are presented as mean±s.e., n = 3, *P<0.05. (c) PTK/ZK inhibited VEGF stimulated Akt pathway. HSCs were treated for 3 h with PTK/ZK where indicated, and were then stimulated with 10 ng/ml VEGF for 10 min. Cell lysates were analyzed for total Akt and phospho-Akt by western blot. The results of a representative study from three experiments are shown.