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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2010 Mar 30;80(2):255–261. doi: 10.1016/j.bcp.2010.03.025

Figure 2.

Figure 2

The lack of NKT cells in CD1d−/− mice did not inhibit halothane metabolism. Female WT and CD1d−/− mice (3 mice/group in panel A and 4 mice/group in panel B) were treated with halothane, and after 8 h, liver homogenates were prepared and used (30 μg/lane) for immunoblot detection of (A) TFA-protein adducts with anti-TFA polyclonal antibodies (1:1000 dilution) and (B) CYPP450 2E1 with anti-2E1 polyclonal antibodies (1:5000 dilution). Molecular mass markers (kDa) are indicated on the right. β-actin served as a loading control (indicated by arrow).