Table 1.

Details of primer pairs used for cloning and qPCR experiments

Gene	Accession No.	Sense Primer (5′-3′)	Tm, °C	Antisense Primer (5′-3′)	Tm, °C	Spanning Exon-Junction	Annealing Temp, °C	Product Size, bp
akirin1(1a)	GQ247756	TTCGTTGTCTCTTTTGAGCAGG	60	CAGTAGAAGAGCAGCCATCTTGTG	61	N/A	58	642
		GTCTCTTTTGAGCAGGCACCAT	61	CGGGGGAGACATCGAGTGTGATG	68	Neither	62	197
akirin1(1b)	GQ247757	TACATCTTCGTTGTCTATTTTGCTCAG	62	TCTAGTGGAGGAAGGGGCAGTAG	62	N/A	59	664
		CATCTTCGTTGTCTATTTTGCTCAGG	63	GTCGGGGGAGACATCGAGTGCG	71	Neither	62	206
akirin1(2a)	GQ247758	CTACATTTTGACCGACACCCC	59	TGGAGATAGGCGAGCAGCAGT	62	N/A	59	675
		CTCAAGGACCATGACGAGAACATCC	66	GACATAGCTGGAGGGTCTGGCG	66	Antisense	62	141
akirin1(2b)	GQ247759	CTAGCATGGCGTGCGGAG	61	GCAGGTAGCTTACTGTTAAGAGTTCA	59	N/A	59	574
		GAGAACATAGACTTACCCCAGAGCAGA	64	GGTCCCTCTTCATAGACACTCCTGATG	66	Both	64	185
akirin2(1a)	GQ247760	ATGGCTTGTGGGGCTACTTTG	62	TCAGGAAACATAGCTAGCAGGTTG	61	N/A	59	555
		TCGTCCAGACTCACAACGGAGCAA	69	GATGGGGAGATGGCACCAGAAGATGTT	71	Both	64	191
akirin2(1b)	GQ247761	TCAGGAAACATAGCTAGCAGGTTG	62	TCAGGAAACATAGCTAGCAGGTTG	61	N/A	59	555
		GCCCCAATGTCTCCAGCCGCTTAC	71	TATTGTGCAGGATTTGCTCGGTTGTG	69	Antisense	64	115
akirin2(2a)	GQ247762	ATCATGGCGTGTGGAGCGAC	64	TCAGGAAACATAGCTAGCAGGTTG	61	N/A	59	546
		CGGTGTCCCCATCATCATCCT	64	TCTCCTCATATTCCTCCCGCACTTTG	69	Neither	64	354
akirin2(2b)	GQ247763	ATCATGGCGTGTGGAGCGAC	64	TCAGGAAACATAGCTAGCAGGTTG	61	N/A	59	549
		GTTTCCCCATCATCCTCCTCATCC	67	GGTCTCCTCATATTCCTCCCGAACTTTA	68	Neither	65	242
IGF-I	GU933431	GTGATGTCTTCAAGAGTGCGATGTG	64	CGCCCCTGTTGCCGCCGAA	74	Sense	61	99
IGFBP5.2	GU933428	GCAGCCTGAGTTTGGACCCT	61	CGTGATGGTTTACACTGCTTGTG	61	Sense	60	151
IGFBP6	GU933435	CGCATAGTGGTGAAATAGAAAAGGC	64	CCCTTGGAGGAACGACACTGCTT	67	Both	60	160
pappa	GU933427	ACCGCAGACAGCGTACTCAAAG	62	TTGTCTCCCATAAAGGGCTCAC	60	Antisense	60	140
stat3	GU933429	CCTTAAAAGTCAGGGTGAGTTGTCC	63	CAGTCAGCCAGCTCGTCATCGG	68	Sense	60	201
mstn1a	NM_001123549	GGGGGCAGGCACATAGGT	60	TCCATGAAGGGTTGCAGTC	56	Antisense	60	211
UBE2H(1)	NM_001165354	ACAGCCCTTTACGATCTCACCAAT	58	TTCTGGATGTACTCTTTGATTTTCTG	59	Neither	59	155
UBE2H(2)	BX080911	AAAGGAGCAAGAGGAGGGAGGTG	65	GAAATGATAGTCCGTGTGGGTGAG	63	Neither	60	136
NF-κB p65	EL548185	AGCATCCCTGGAGAGAAGAGCAA	64	ATGCCCAGGTTTTGAAAACTGTG	63	Antisense	60	217
14-3-3γ1	AY370885	AGCCATGAAATCGGTGACAGAGT	61	CGCTGTAGGACTTCTCAGAGGACT	63	Sense	61	391
14-3-3γ2	AY370886	GCTGCCATGAAATCGGTAACAG	62	TTGTAAGCCTTCTCTGATGATTCG	61	Sense	61	391
14-3-3β2	NM_001124469	CTTCTATGAAATCCTCAACAACCCA	62	CCGATGTCCACAGAGTCAGGTGTC	64	Antisense	61	160
rps13	BT059859	CCCTCTCAGATCGGTGTGATCC	63	TCCTTGTCCTTTCTGTTCCTCTCC	63	Both	60	193
rps29	NM_001139600	GGGTCATCAGCAGCTCTATTGG	61	AGTCCAGCTTAACAAAGCCGATG	63	Antisense	60	167
rpl4	BT057966	CCTTCAGAAACATCCCTGGTATCAC	63	GGGCAGATTGTAGTCTACCTTGAGAG	62	Both	62	182
rpl13	BT060300	CGCTCCAAGCTCATCCTCTTCCC	68	CCATCTTGAGTTCCTCCTCAGTGC	64	Antisense	62	79

Primer pairs in italic type were used for cloning; primer pairs in roman type were used for quantitative real-time PCR (qPCR). Accession no, NCBI GenBank accession number related to the sequence; Tm, primer melting temperature, calculated with NetPrimer (http://www.premierbiosoft.com), stated to the nearest 0.5°C; product size, product size for Arctic charr (Salvelinus alpinus). Spanning Exon-Junction column indicates whether the sense, antisense, both, or neither primer is predicted to span an exon-exon junction, based on conservation in teleost, bird, and mammal orthologs; N/A, not applicable. Details of primer sequences for IGF-II, IGFBP2a, IGFBP2b, IGFBP4, IGF-IR1a, IGF-IR1b, β-actin and RNA Pol-II were published in Ref. 8. Details of primer sequences for murf1 and mafbx were published in Ref. 9.