Figure 4. Absence of the TCRβ chain does not affect phosphorylation of components of the JAK/STAT signalling pathway.

Western blotting on cell lysates obtained from IFNα-stimulated Jurkat and JRT3-T3.5 cells using (a) anti-phospho(Tyr¹⁰⁵⁴/Tyr¹⁰⁵⁵)-Tyk2 antibody, (b) anti-phospho(Tyr⁷⁰¹)-STAT1 antibody, (c) anti-phospho(Tyr⁷⁰⁵)-STAT3 antibody and (d) anti-phospho(Tyr⁶⁹⁴)-STAT5 and anti-STAT5 antibodies. Total ERK1/2 was used to demonstrate equal loading