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. 2010 Jun 21;5(6):e11235. doi: 10.1371/journal.pone.0011235

Figure 3. The c-fos conserved intronic region drives luciferase expression and responds to the CREB and AP-1 pathways.

Figure 3

A. 5′ part of mouse c-fos gene (up) and our reporter construct (bottom), fiL (fos intron Luciferase). B. The fos intronic region suffices to drive Luciferase activity in NIH3T3 cells in transient transfection. The promoterless pGL2 basic vector background activity is shown as a control. Error bars represent standard deviation, n = 7. C. The fIL construct responds to the calcium and cAMP pathways, but not to PMA. NIH3T3 transfected with fiL were treated with the indicated drugs. Error bars correspond to standard deviation, n = 6. D. Co-transfection with CREB or AP-1 expression vectors stimulates fIL promoter activity. Error bars correspond to standard deviation, n = 5. Statistical analysis of variance (ANOVA) was performed (*: p<0.05; **: p<0.01 relative to control).