Fig. 2.
Strain YYC1273 (cfa::kan) carrying either the vector plasmid pBAD24 or the AasS encoding plasmid pYFJ85 was grown overnight at 37oC on each of the [1-14C]-labeled short chain fatty acids shown at 15 μM. The acyl chains were analyzed as their methyl esters by argentation chromatography. When added arabinose was present at 0.02%. Sat, saturated fatty acid esters; Cvc, cis-vaccenic acid (cis-11-octadecenoic acid); Pol, palmitoleic acid (cis-9-hexadecenoic acid, Δ9-C17, cis-9-heptadecenoic acid and Δ7-C15, cis-9-pentadecenoic acid. Identification of the fatty acids were done using authentic standards as described previously (19) excepting the odd chain unsaturated acids which were identified by reference to the prior literature (32, 33).