Table 1.
E. coli K-12 strains and plasmids used in this work.
| Strain or Plasmid | Relevant Characteristics | Source or Reference |
|---|---|---|
| Strains | ||
| UB1005 | metB1 relA1 spoT1 gyrA216 lr l | Lab collection |
| CL81 | UB1005, attHK022::(plsX’fabH, Spcr Strr) | (10) |
| CL111 | UB1005, attHK022::(plsX’fabH, Spcr Strr), fabH::Kan | (10) |
| YFJ248 | CL111/pBAD24 | This work |
| YFJ249 | CL111/pYFJ85 | This work |
| CAG12094 | MG1655, zcb-3059::Tn10 | (28) |
| CAG18466 | MG1655, zcb-282::Tn10 | (28) |
| YFJ260 | fabH::Kan, zcb-3059::Tn10, Spcs Strs/pYFJ85 | This work |
| YFJ262 | fabH::Kan, zcb-282::Tn10, Spcs Strs/pYFJ85 | This work |
| YFJ293 | UB1005/pBAD24 | This work |
| YFJ294 | UB1005/pYFJ85 | This work |
| YFJ303 | YFJ294 fabH::Kan | This work |
| YFJ304 | YFJ293 fabH::Kan | This work |
| YFJ305 | UB1005 fabH::Kan | This work |
| GRT26 | MC1061 cfa::Cm | Lab collection |
| YFJ315 | YFJ303 cfa::Cm | This work |
| YFJ316 | YFJ304 cfa::Cm | This work |
| YFJ317 | YFJ305 cfa::Cm | This work |
| CHC41 | UB1005, cfa::kan | This work |
| K19 | fadE62 | (23) |
| K27 | fad88 | (23) |
| CAG18483 | fadL771::Tn10 | (28) |
| CAG18496 | fadA 101::Tn10 | (28) |
| JWC255 | fadE62 cfa::kan | This work |
| YYC1273 | cfa::kan | (9) |
| Plasmids | ||
| pBAD24 | Expression vector with pBAD promoter | (8) |
| pCR2.1-TOPO | TOPO TA cloning vector | Invitrogen |
| pYFJ82 | aasS PCR amplified and inserted into pCR2.1-TOPO | This work |
| pYFJ85 | aasS fragment of pYFJ82 inserted in to the NcoI/HindIII sites of pBAD24 | This work |
| pMON5839 | Expression vector | (29) |
| pBB277 | Saccharomyces cerevisiae FAA1 gene in pMON5839 | (29) |