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. Author manuscript; available in PMC: 2010 Jun 21.
Published in final edited form as: Kidney Int. 2009 Apr 8;76(1):63–71. doi: 10.1038/ki.2009.98

Figure 1. Phosphorylation of Stat3 in the kidney.

Figure 1

(a) Immunostaining of total and phosphorylated Stat3 in kidney sections. Immunohistochemistry was performed on paraffin sections of kidneys from SA/+ and SA/– mice with and without STZ-induced diabetes (SA/+, SA/+ DM, SA/–, and SA/– DM) using rabbit polyclonal anti-total Stat3 (T-Stat3) and anti-phospho-Stat3 antibodies (p-Stat3). The staining for T-Stat3 in SA/+ was more than in SA/– mice. Representative immunostaining results are shown. (b) Western blot of T-Stat3 and p-Stat3 in isolated glomeruli. Isolated glomeruli from diabetic and non-diabetic SA/– and SA/+ mice were lysed in lysis buffer containing protease and phosphatase inhibitors. Western blot was performed using anti-total Stat3, anti-phospho-Stat3 (Cell Signaling Technology), and anti-β-actin (Sigma) antibodies. Representative blots of three independent experiments are shown.

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