Table 2.
Semiquantitative assessment of staining
Type IV collagen (%) | TGF-β (%) | Ki67 | CD68 | |
---|---|---|---|---|
SA/+ | 7.8 ± 1.3 | 4.4 ± 1.1 | 1.34 ± 2.46 | 0.35 ± 0.50 |
SA/+ DM | 26.2 ± 3.2 | 15.3 ± 3.5 | 7.87 ± 2.52 | 3.55 ± 0.68 |
SA/– | 8.2 ± 1.4 | 5.1 ± 1.6 | 1.25 ± 1.56 | 0.18 ± 0.12 |
SA/– DM | 9.3 ± 2.4* | 5.5 ± 1.2* | 3.00 ± 0.36* | 0.52 ± 0.11* |
TGF-β, transforming growth factor-β.
Semiquantitative assessment of staining: intensity of immunostaining was quantified as described in the Materials and Methods section. For type IV collagen and TGF-β, % of positive staining area per glomerular cross-section was measured by MetaMorph software. For Ki67 and CD68, number of positive cells per glomerular cross-section was counted.
N=6
P < 0.01 as compared with SA/+ DM.