Figure 2. Expression of intermediate and late SMC markers after one week of differentiation.

RT-PCR for calponin, caldesmon, and MHC was performed for 4 patient cell lines after differentiation in ANG, SPC or TGFβ1 for one week. Expression of calponin was seen with all 3 differentiating agents in patient 4 only. Up-regulation of caldesmon was seen in patients 1 and 3 only. No MHC expression was seen with any cell lines at one week.