Figure 4.
Effect of abscisic acid (ABA) and rosiglitazone combination on gene expression in lipopolysaccharide (LPS)-treated stromal vascular cells (SVCs). SVCs from abdominal white adipose tissue were isolated from db/db mice fed high-fat control diet for 60 days. Cells were seeded into 24-well plates at 1 × 106 cells/well and treated with LPS (100 ng/mL) with and without ABA (10 μM), Ros (1 μM), or ABA and Ros (ABA/Ros). The relative expressions of genes peroxisome proliferator activated receptor γ (PPAR γ) (A), CCL17 (B), monocyte chemoattractant protein 1 (MCP-1) (C), and mannose receptor (D) were calculated as a ratio to the housekeeping gene β-actin. Data are represented as mean ± standard error. Points with an asterisk are significantly different the control treatment (P<0.05).