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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Clin Nutr. 2010 Mar 5;29(5):646–653. doi: 10.1016/j.clnu.2010.02.003

Figure 5.

Figure 5

Effect of abscisic acid (ABA) and rosiglitazone (Ros) combination on PPAR γ transactivation in 3T3-L1 pre-adipocytes and RAW 264.7 macrophages. 3T3-L1 preadipocytes and RAW 264.7 macrophages were seeded into the lower and upper chambers, respectively, of a 24-transwell plate before being transfected with a pTK.PPRE3x luciferase reporter plasmid driven by the PPRE-containing Acyl-CoA oxidase promoter and a pRL control plasmid. Cells were treated for 24 with ABA (10 μM), Ros (1 or 10 μM), or their combination. Luciferase activity was normalized to pRL activity in the cell extracts and relative luciferase activity was calculated a ratio of the activity in the treatment wells to control wells. Data are represented as mean ± standard error. Points with an asterisk indicate that a treatment is significantly different from control, non-ABA treated well (P<0.05).