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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Cancer Biol Ther. 2010 Apr 1;9(7):526–536. doi: 10.4161/cbt.9.7.11116

Figure 1.

Figure 1

Figure 1

Figure 1A and B. Ad.mda-7 lethality is enhanced by OSU-03012. (A) GBM6, GBM12 and GBM14 cells were infected with empty vector control virus (Ad.cmv) or with virus to express MDA-7/IL-24 (Ad.mda-7) and 12 h after infection treated with vehicle (DMSO) or OSU-03012 (OSU, 1 μM). Forty eight h after infection cells were isolated and cell viability was determined by trypan blue exclusion assay (±SEM, n = 3). (B) GBM6 cells were plated as single cells in sextuplicate and 12 h after plating infected with Ad.cmv or Ad.mda-7 and 12 h after infection treated with vehicle (DMSO) or OSU-03012 (OSU, 1 μM). Forty eight h after infection the growth media was removed and replaced with new media lacking drugs. Colonies of >50 cells were permitted to form over the following ~20 days, followed by fixing, staining and counting (±SEM, n = 3).

Figure 1C and D. Ad.mda-7 lethality is enhanced by OSU-03012. (C) GBM6 cells were infected with Ad.cmv or Ad.mda-7 and 12 h after infection treated with vehicle (DMSO) or OSU-03012 (OSU, 1 μM). Twenty four h and 48 h after infection cells were isolated and SDS PAGE performed to determine the expression of BCL-XL and MCL-1, and the phosphorylation of ERK1/2, p38 MAPK, JNK1-3, AKT (S473) (n = 2). (D) GBM6 cells were infected with Ad.cmv or Ad.mda-7 in combination with viruses to express XIAP, BCL-XL, dominant negative caspase 9 or c-FLIP-s, and 12 h after infection treated with vehicle (DMSO), or OSU-03012 (OSU, 1 μM). Forty-eight h after infection cells were isolated and cell viability was determined by trypan blue exclusion assay (±SEM, n = 3).