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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Nutr Biochem. 2009 Oct 28;21(10):906–913. doi: 10.1016/j.jnutbio.2009.07.002

Figure 3. Only galactosidase that hydrolyzed the α-1→3-galactosidic bond produced decline in IL-8 production.

Figure 3

Figure 3

A. Pre-treatment of carrageenans by α-1→6-galactosidase (20 U/L) had no effect on IL-8 secretion in the NCM460 cells. Data are the mean ± S.D. and are representative of 9 independent biological experiments with technical replicates of each. (CGN=carrageenan)

B. Pre-treatment by β-1→4-galactosidase (20 U/L) of κ-carrageenan and short κ8 CGN had no effect on IL-8 secretion in the NCM460 cells. Data are the mean ± S.D. and are representative of 9 independent biological experiments with technical replicates of each. (Gal=galactosidase)

C. Pre-treatment of carrageenans by α-1→(3,6)-galactosidase (20 U/L) produced significant declines (p=0.03 for κCGN; p=0.003 for κ8CGN; p=0.008 for ιCGN by paired t-tests) in IL-8 secretion in the NCM460 cells, indicating the importance of the Gal-α-(1→3)-Gal epitope for the carrageenan-induced IL-8 response. Data are the mean ± S.D. and are representative of 14 independent biological experiments with technical duplicates for κ-CGN and n=6 for short κ8CGN and ι-CGN.

D. IL-8 secretion increased following exposure to increasing concentrations of α-1→(3,6)-galactosidase (20 U/L to 200 U/L) in the NCM460 cells (p<0.001, 1-way ANOVA with Tukey-Kramer post-test and p<0.0001 for linear trend). Data are the mean ± S.D. of 3 independent biological experiments with technical duplicates of each. (CGN=carrageenan)