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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Nutr Biochem. 2009 Oct 28;21(10):906–913. doi: 10.1016/j.jnutbio.2009.07.002

Figure 5. Demonstration of κCGN degradation products following enzymatic digestion by PAGE.

Figure 5

Digestion products of heparin are presented as molecular weight standards (Std). κ-CGN of high molecular weight without exposure to enzymes is in lane 1. Lane 2 is κ-CGN digested by α-1→(3,6)-galactosidase; lane 3 is κ-CGN un-digested by α-1→6-galactosidase; lane 4 is κ-CGN digested by κ-carrageenase; and lane 5 is κCGN digested by a combination of κ-carrageenase and α-1→(3,6)-galactosidase. Lower molecular weight products are shown in lanes 4 and 5 than in lane 2. In the experiments with the NCM460 cells, κ-carrageenase was used at a ten-fold lower concentration relative to the amount of carrageenan than in this analysis.