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HNSCC cells and keratinocytes affect monocyte phenotype in vitro. Monocytes from Donor 1 (a) and Donor 2 (b) were cultured alone or co-cultured with keratinocytes or HNSCC cells for up to 3 days and evaluated by flow cytometry for cell-surface phenotype, as described in the “Materials and Methods”. The results are representative of at least three independent experiments for each donor.
