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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Atherosclerosis. 2010 Feb 6;211(1):90–95. doi: 10.1016/j.atherosclerosis.2010.01.046

Figure 2. Characterization of SAP in atherosclerotic aortic intima.

Figure 2

A and B. Gel filtration analysis of SAP in atherosclerotic lesions. The sequential intimal extracts from an advanced atherosclerotic aorta by CaCl2-TBS, EDTA-TBS and collagenase digestion were applied to FPLC equipped with a Superose 6 HR 10/30 column. The fractions were monitored by UV A280nm, and SAP in each fraction was detected by electroimmunoassay and the presence of SAP was indicated by horizontal bar (A). The collagenase digested extract was also reduced with 2mM DTT and subjected to FPLC analysis (B). C. SDS-PAGE-Western blot analysis of SAP in atherosclerotic or normal aorta. The sequential intimal extracts from an advanced atherosclerotic aorta or a normal aorta were applied to reduced SDS-PAGE and the SAP in sequential extracts was analyzed by Western blot with anti-SAP antiserum.