Figure 4.

RBP1 regulates Runx2 through the Runx2 P2 promoter. Runx2-specific primer pairs designed to transcripts arising from either the Runx2 P1 promoter (open bars) or the Runx2 P2 promoter (closed bars) were used in a real-time QPCR analysis from RNA isolated from the CalOB-Control or CalOB-ΔRBP1 cell lines. The data are expressed as Runx2 expression relative to a TBP controls and normalized to the day 0 CalOB-Control cell line. The data represent the mean ± standard deviation. The asterisks represents statistical significance at the p < 0.01 level (ANOVA).