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P2 residues in peptides released from Hb following digestion by Ov-CB-1 and Ov-CF-1 were determined by nanoLC-ESI-MS/MS analysis of digest samples. The frequency by which amino acids occur at the P2 positions of Hb α- and β-chains (converted to a percentage of the total) are plotted for the 15 min reactions shown in (Figure 6A).
