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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2009 Apr 21;8(5):1008–1014. doi: 10.1158/1535-7163.MCT-08-0706

Figure 3.

Figure 3

γ-H2AX induction by NSC 725776 and NSC 724998 in human colon cancer HT29 cells. A) Rapid induction of γ-H2AX foci after 1 h treatment with 1 µM of CPT, NSC 725776 or NSC 724998. B) Persistent γ-H2AX signal induced by indenoisoquinolines. After 1 h drug treatments, HT29 cells were further incubated in drug-free medium for 24 h. C) Representative image of a single cell with typical γ-H2AX damage foci. D) Representative image of a single cell with diffuse pan-nuclear staining, which typically develops several hours after drug exposure. Fixed cells were stained with mouse anti-γ-H2AX antibody and goat anti-mouse antibody conjugated with AlexaFluor 488 (green). Nuclei were stained with PI (red).