Table 1.

Summary of Disease in the Cervix and Vagina of Mice After 9 Months with Estrogen

	Mouse Genotype	Severity of Disease^a
	Mouse Genotype	H	CIN1	CIN2	CIN3	MIC	LIC
cervix	*NTG*	17	3	1		2
	*E6AP*^−/−	2	11	2	1	3
	*K14E6*^WT^b	3	9	7	8	5	7
	*K14E6^WTE6AP*^−/−^c	1	12	2		1

vagina		H	VAIN1	VAIN2	VAIN3	MIC	LIC

	*NTG*	16	7
	*E6AP*^−/−	1	18
	*K14E6*^WT^b	9	12	15	2	1
	*K14E6^WTE6AP*^−/−^c	5	9	2

Cohorts of female mice of the indicated genotypes were treated with estrogen for 9 months and the reproductive tracts from every mouse was scored for the worst state (severity) of cervical (top half) or vaginal (bottom half of table) disease. The overall severity of disease between different genotypes was compared using a two-sided Wilcoxon-rank sum test. Abbreviations: H, hyperplasia; CIN, cervical intraepithelial hyperplasia; MIC, microinvasive cancer; LIC, large invasive cancer; VAIN, vaginal intraepithelial neoplasia.

The shift in the severity of disease in K14E6^WT transgenic mice is highly significant relative to NTG and E6AP^−/− mice (p ≪ 0.001).

Loss of E6AP results in a highly significant reduction in the severity of disease in K14E6^WTE6AP^−/− in the cervix (p≪0.001) and a significant reduction in the vagina (p = 0.05) relative to K14E6^WT mice.