Figure 2.

Analysis of ITC measured enthalpy vs. molar ratio. (A) As dendrimers were injected into the lipids, initially there were abundant lipids. The heat release per dendrimer increased as the lipid vesicles aggregated until the stoichiometric ratio of n_A. The heat release was equal to ΔH⁰ between n_A and n_L. At the molar ratio n_L, the lipids became scarce and each injected dendrimer released less heat. At the molar ratio n_D, all the lipids were consumed and no further dendrimer-lipid interaction was observed. (B, C) The mixture of dendrimers with lipids vesicles resulted in aggregation as the dendrimers bind to the membranes. Further addition of dendrimers resulted in the formation of dendrimer-lipid complexes of well defined stoichiometry and continued flocculation and/or aggregation.