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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Res. 2010 Jun 8;70(12):5074–5084. doi: 10.1158/0008-5472.CAN-09-4094

Figure 2. LMW-E overexpression leads to mitotic defects.

Figure 2

A. Cells were treated with doxycycline (1 μg/ml) for 24 h, fixed, and stained for β-tubulin (green), γ-tubulin (red), or DNA (blue) and analyzed by IF microscopy. B, Quantification of spindle defects in 200 mitotic cells 200 cells/experiment from ten independent experiments. Data are shown as the percentage of cells with mitotic defects and error bars indicate SD. *P < 0.001. C, Pictorial presentation of various mitotic errors (micronuclei, chromosomes missegregation, anaphase bridges, cytokinesis failure) in LMW-E–overexpressing cells. D, Quantification of mitotic errors in control, EL-expressing, and LMW-E–expressing cells. Graphs indicate the percentage of cells with micronulei, metaphase chromosome missegregation, anaphase bridges, and cytokinesis failure (mean +/− SD, n = 3). *P < 0.01, **P <0.001.