Figure 10.
Cells harvested from spheres that formed in air-liquid interface cultures incorporate into the regenerating OE of the host after transplantation. (A–C) (D–F) Adjacent sections through a single graft-derived cluster 11 days post-transplantation. This cluster contains donor-derived (green) neurons (thin arrows) double labeled by Tuj-1 staining (red in B–C), sustentacular cells (thin open arrows) and HBCs (triangles) double labeled by bBS-I and SUS-4 staining (red in E–F) and ciliated respiratory epithelial cells (thick open arrows in A–F). (G–I) Another clone consists mainly of sustentacular cells (thin open arrows) positioned above the layer of neurons and marked by thin foot processes extending toward the basal lamina. Ciliated respiratory epithelial cells (thick open arrows in G–I) are also seen in this clone. The images were obtained with a confocal microscope. Scale bars in I is 20 µm and apply to A–H.