Epithelial islands develop from adult olfactory epithelium in submerged cultures. Bright field photomicrographs showing the two-dimensional submerged cultures of dissociated cells from the olfactory epithelium (OE) from normal (A), MeBr-exposed (B; at 2 days postlesion), and bulbectomized (C; at 5 days postlesion) mice grown on matrigel in serum-rich NIC medium. In normal and MeBr-exposed OE cultures, epithelioid islands are seen (asterisks in A and B) as well as scattered, elongated, spindle-shaped cells around the islands. In bulbectomized OE culture, the spindled cells are much more prevalent (C). Scale bar in A is 50 µm, and applies to B and C.