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. Author manuscript; available in PMC: 2010 Jun 21.
Published in final edited form as: Exp Neurol. 2008 Jul 22;214(1):25–36. doi: 10.1016/j.expneurol.2008.07.012

Figure 4.

Figure 4

TuJ-1(+) cells are present in the monolayer cultures of MeBr-lesioned OE grown on laminin in serum-rich NIC medium (A–D) and serum-free KGM (E–H). The TuJ-1(+) cells are elongated cells present surrounding epithelioid islands (asterisks in A and E–H). Note the difference in abundance of TuJ-1(+) cells in the two media conditions. In serum-rich condition, some TuJ-1(+) cells are heavily stained and clearly bipolar (double arrows in B, C and D), suggesting neuronal differentiation, while others are some weakly stained cells, and are usually spindle-shaped (arrowheads in B and D). In serum-free condition, such heavy labeled cells are scarce, but clearly neuronal-like in shape. Indeed, the leading process of some TuJ-1(+) cells closely resembles growth cones (thin arrow in E and H), particularly when found around the edges of the islands (asterisks). Note that not all scattered cells are TuJ-1(+). Scale bar in H is 50 µm for A and 25 µm for B–H.