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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Cancer Cell. 2010 Jun 15;17(6):609–621. doi: 10.1016/j.ccr.2010.04.012

Figure 3. Amino Acids within the RD2 region are necessary for MLL interaction with the PAF complex.

Figure 3

A) Schematic of Myc-tagged CxxC-RD2-AF9 constructs made with serial deletions of the RD2 region. The first and last MLL amino acid retained in the expression constructs are indicated. All constructs include the AF9 fusion partner at the C-terminus and Myc tag at the N-terminus. The Cx-1154-AF9 through Cx-1357-AF9 constructs are named according to the last MLL residue retained in the fusion protein. B) Myc-tagged RD2 deletion constructs described in A were expressed in 293 cells followed by immunoprecipitation. The Myc-tagged MLL deletion proteins and associated PAF complex components were detected by western blot with the indicated antibodies. C) Schematic of a second set of deletion constructs made through the RD2 region that also delete the pre-CxxC domain. Cx-del-1209-AF9 through Cx-del-1357-AF9 were made by deleting amino acids 1115 through 1179 of MLL. The amino acids included in each construct are indicated. D) Myc tagged constructs described in C were expressed in 293 cells, immunoprecipitated and detected as described in B.