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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Cancer Res. 2010 May 25;8(6):833–843. doi: 10.1158/1541-7786.MCR-09-0400

Figure 4.

Figure 4

Spry4 re-expression induces a more epithelial phenotype in NSCLC cells. (A) 5,000 cells from H157 and H2122 stably expressing Spry4 or an empty vector control were seeded in media with 4% matrigel on top of a 1:1 matrigel and media base layer. Cell morphology was recorded on day 5. Data is representative of triplicate experiments. (B) Western blot analysis of E-cadherin in cell lysates from H157 and H2122 cell lines with stable Spry4 expression compared to an empty vector control. Loading control is β-actin and data are representative of triplicate experiments. (C) QPCR for KRT8, KRT18, and Vimentin in H157 and H2122 cell lines stably expressing Spry4 compared to an empty vector control. Results are the average of triplicate experiments and are normalized to GAPDH.