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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Brain Res. 2010 Feb 6;1323:119–126. doi: 10.1016/j.brainres.2010.02.002

Figure 2.

Figure 2

Photomicrographs of neurons in a section through the tuberal region of the hypothalamus of animal C37; the section was processed using immunofluorescence to dual-localize MCH and rabies virus. A–B: photomicrographs taken using a 20X objective; column 1 shows MCH immunopositivity, column 2 shows rabies immunopositivity, and column 3 shows the combined immunopositivity for both antigens. Arrows denote examples of double-labeled neurons; note that no double-labeled cells are present in B. C: a montage of photomicrographs taken using a 4X objective, showing immunopositivity to both rabies and MCH. Boxes in panel C show the locations of the neurons depicted at higher magnification in A–B. Calibration bars represent 50 μA in AB and 1 mm in C.