Fig. 4.

Targeting of Dnmt3b to deregulated germ-line genes requires E2F6 binding. (A) Co-immunoprecipitation of Dnmt3b with E2F6 from transiently transfected HEK-293 cells with Dnmt3b, E2F6, or both expression vectors, using specific antibodies against Dnmt3b and E2F6. Precipitated proteins were analyzed by Western blotting and revealed using E2F6- and Dnmt3b-specific antibodies. (B) ChIP assays performed on chromatin prepared from WT and E2F6^−/− MEF, using Dnmt3b- and E2F6-specific antibodies, followed by RT-PCR analysis using primers amplifying segments in the proximal promoters of the indicated genes. IN, input.