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Recruitment kinetics of EGFP-APLF and PBZ mutants following laser-induced DNA damage. (A) Live cell imaging analyses of U2OS cells stably expressing EGFP-APLF proteins (full-length) as indicated following laser microirradiation and time-lapse imaging over 5 min. (B) Quantitation of the recruitment kinetics in A. The average relative fluorescence (± SE) of EGFP-APLF from at least 10 cells from three independent experiments was plotted over the 5-min time course.
