Fig. 2.

Characteristics of naïve hESC lines. (A) Scheme for reverting hESCs to generate naïve hESCs. Representative images of WIBR3 hESCs at different stages of the reversion process in PD/CH/LIF/FK. p, passage number. Magnifications of captured images are indicated. (B) Single-cell cloning efficiency of different pluripotent stem cell lines as determined by the number of wells containing Nanog+ colonies after 7 days. (C) Estimated cell doubling time. Plated cells were plated in triplicates and counted at 1, 4, and 7 days after plating, and increase in cell number was used to extrapolate average doubling time. Error bars represent SD, and P values using Student's t test indicate significant difference in the average of hESC/hiPSC lines in comparison with the average of naïve hESC/hiPSC lines.