Fig. 2.

The FV(a)-dependent increase in the activation of FXI by thrombin enhances the coagulation reaction. FXI (30 nM) was activated with thrombin in the presence of 10 μM PS:PC:PE and vehicle or FVa (30 nM) for 10 min at 37 °C, after which reactions were stopped with hirudin (10 U/mL). Thrombin and FVa alone served as control. (A) Coagulation was initiated with diluted samples as described in Materials and Methods. Data are shown as mean ± SD for data from at least three experiments. (B) Thrombin generation was measured with the Technothrombin TGA kit as described in Materials and Methods. Graph shows a representative experiment. *P < 0.05.